Our lab has worked for several years to create a bank of patient-derived cultures and xenografts from neurosurgical specimens.
We have developed a bank of over 45 patient-derived neurosphere cultures from adult glioblastoma and pediatric high-grade gliomas for in vivo and in vitro research on tumor stem cell biology, tumor cell invasion, and tumor genomics. These have formed the basis of all of our ongoing research projects on pre-clinical therapeutics. We are also using these models in xenograft models and human brain organoid experiments and adapting them to epigenomics and single cell RNA-seq profiling experiments designed to assess the mechanism of action of therapeutic agents under study in our lab.
An excellent example of our work in this area is our ongoing research on targeting the YAP and TAZ transcription factors in glioblastomas:
Vigneswaran, K., N.B. Boyd, S. Lallani, S.-Y. Oh, A. Boucher, S. Neill, J.J. Olson, R.D. Read. 2020. YAP/TAZ transcription factors create therapeutic vulnerability to verteporfin in EGFR mutant glioblastoma. Clinical Cancer Research. Nov 10;clincanres.0018.2020.doi: 10.1158/1078-0432.CCR-20-0018.
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